Tabriz University of Medical Sciences Journal of Cardiovascular and Thoracic Research 2008-5117 16 1 2024 03 13 Acellular carotid scaffold and evaluation the biological and biomechanical properties for tissue engineering 28 37 10.34172/jcvtr.32899 EN Farina Rashidi https://orcid.org/0009-0001-5852-5980 Mehdi Mohammadzadeh Arash Abdolmaleki https://orcid.org/0000-0003-2858-4098 Asadollah Asadi Mehrdad Sheikhlou Journal Article 10.34172/jcvtr.32899 2023 06 28 2024 02 10 Introduction: The issues associated with the limitation of appropriate autologous vessels for vascular reconstruction via bypass surgery highlight the need for new alternative strategies based on tissue engineering. The present study aimed to prepare decellularized scaffolds from ovine carotid using chemical decellularization method. Methods: Ovine carotid were decellularized with Triton X-100 and tri-n-butyl phosphate (TnBP) at 37 °C. Histological analysis, biochemical tests, biomechanical assay and biocompatibility assay were used to investigate the efficacy of decellularization. Results: Decellularization method could successfully decellularize ovine carotid without leaving any cell remnants. Scaffolds showed minimal destruction of the three-dimensional structure and extracellular matrix, as well as adequate mechanical resistance and biocompatibility for cell growth and proliferation. Conclusion: Prepared acellular scaffold exhibited the necessary characteristics for clinical applications.