Hami Asadi
1, Ali Akbar Abolfathi
1, Reza Badalzadeh
2, Maryam Majidinia
3, Alireza Yaghoubi
2, Maryam Asadi
4, Bahman Yousefi
3,4,5*1 Department of Biological Science, Ahar Branch, Islamic Azad University, Ahar, Iran
2 Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
4 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5 Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract
Introduction: Serum amyloid-A (SAA) and protein carbonyl group are rigorously related with cardiovascular diseases (CVDs) as a sensitive marker of an acute inflammatory state and as an important index of oxidative stress, respectively. Moreover, diet is one of the main factors that canmodify cardiovascular risks. Therefore, this study aimed to investigate the effects of Ramadanfasting on SAA and protein carbonyl group levels in patients with CVDs.Methods: Twenty-one patients (21 male; mean age 52±9 years old) with CVDs (coronaryartery disease, cerebrovascular, or peripheral arterial diseases) were participated in this study.Biochemical parameters were measured in patients 2 days before and 2 days after Ramadanfasting. SAA levels were assessed using enzyme-linked immunosorbent assay and Cayman’sprotein carbonyl colorimetric assay was provided for measuring protein carbonyl groups.Results: According to the findings of the study, post-Ramadan levels of inflammatory biomarker,SAA was decreased significantly in patients with CVDs in comparison with the baseline beforefastingvalues (16.84±8.20 vs. 24.40±6.72 μg/ml, P = 0.021). In addition, Ramadan fastingsignificantly reduced the levels of protein carbonyl group in patients as compared with those ofbaseline values (33.08±15.31 vs. 43.65±16.88 nmol/ml, P = 0.039).Conclusion: Ramadan fasting has impressive effects on modulating CVDs by decreasinginflammation and oxidative stress markers. However, to get a clear conclusion with more results,further investigation is warranted.