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Submitted: 17 Apr 2013
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J Cardiovasc Thorac Res. 2013;5(2):61-65.
doi: 10.5681/jcvtr.2013.013
PMID: 24251013
PMCID: PMC3825387
  Abstract View: 510
  PDF Download: 676

Original Article

Oxidative Versus Thrombotic Stimulation of Platelets Differentially activates Signalling Pathways

Pouran Karimi 1 * , Nadereh Rashtchizadeh 2

1 Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz, Iran
2 Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz, Iran

Abstract

Introduction: Atherosclerosis is one of the inflammatory underlying disease associated by oxidative stress and thrombotic agents. This study aimed to evaluate the potential role of cupper oxidized low-density lipoprotein (OxLDL) and thrombin for inducing mitogen activated protein kinases (MAPKs) in platelets. Methods: Phosphorylation of P38MAPK, Jun N-terminal Kinase (JNK), and Extracellular signal-regulated kinases (ERK1/2) and P-selectin expression were determined in lysates of washed human platelets pretreated with low doses of thrombin and cu2+-OxLDL By Enzyme-linked immunosorbent assay (ELISA). Pharmacological inhibition was performed by SB203580, PD980559 and SP6000125 for P38MAPK, ERK1/2 and JNK activity, respectively. The ratio of phosphorylated to total protein was used for normalizing the phospho proteins contents of cells. Results: OxLDL and thrombin significantly and differentially increased P-selectin expression (P<0.05), P38MAPK (P<0.05) and c-JNK (P<0.05) and ERK1/2 (P<0.05) phosphorylation in platelets. SB 203580 and SP6000125 significantly decreased P-selectin expression in both oxidative (P<0.05) and thrombotic (P<0.05) activated platelets. Conclusion: Our results indicated that MAPK inhibitors can reduce atherothrombotic events via alterations in P-selectin expression suggesting that these inhibitors may be useful in the inhibition of atheroma development.
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