Submitted: 10 Mar 2016
Revision: 28 Jun 2016
Accepted: 11 Jul 2016
ePublished: 26 Sep 2016
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

J Cardiovasc Thorac Res. 2016;8(3): 102-106.
doi: 10.15171/jcvtr.2016.22
PMID: 27777694
PMCID: PMC5075357
Scopus ID: 85026517792
  Abstract View: 1341
  PDF Download: 722

Original Article

A reversal of age-dependent proliferative capacity of endothelial progenitor cells from different species origin in in vitro condition

Mehdi Hassanpour 1, Omid Cheraghi 2,3, Vahid Siavashi 4, Reza Rahbarghazi 3*, Mohammad Nouri 3*

1 Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
3 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


Introduction: A large number of cardiovascular disorders and abnormalities, notably accelerated vascular deficiencies could be related to aging changes and increased length of life. During the past decades, the discovery of different stem cells facilitates ongoing attempts for attenuating many disorders, especially in vascular beds. Endothelial progenitor cells (EPCs) are a subtype of stem cells that have potent capacity to differentiate into mature endothelial cells (ECs). However, some documented studies reported an age-related decline in proliferation and function of many stem cells. There is no data on aging effect upon proliferation and morphological feature of EPCs.
Methods: To show aging effect on EPCs proliferation and multipotentiality, bone marrow samples were provided from old and young cases in three different species; human, mouse and dog. After 7 days of culture, the cell morphology and clonogenic capacity were evaluated. We also calculated the mean number of colonies both in bone marrow samples from old and young subjects. To confirm the cell phenotype, isolated cells were immune-phenotyped by a panel of antibodies against Tie-2, CD133 and CD309 markers.
Results: Our results showed that EPCs exhibited prominent spindle form in all bone marrow samples from young cases while the cell shape became more round by aging. Notably, the number of colonies was reduced in aged samples as compared to parallel young subject samples (P < 0.05). We also detected that the expression of endothelial related markers diminished by aging.
Conclusion: The results of this study suggest that the age-related vascular abnormalities could be presumably related to the decline in stemness capacity of EPCs.
Keywords: Endothelial Progenitor Cells, Human, Mouse, Dog, Aging
First Name
Last Name
Email Address
Security code

Abstract View: 1341

Your browser does not support the canvas element.

PDF Download: 722

Your browser does not support the canvas element.