Submitted: 14 Oct 2018
Accepted: 26 Jan 2019
First published online: 16 Feb 2019
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J Cardiovasc Thorac Res. 2019;11(1):43-47.
doi: 10.15171/jcvtr.2019.07
  Abstract View: 44
  PDF Download: 40

Original Article

Different expression of Micro RNA-126, 133a and 145 in aorta and saphenous vein samples of patients undergoing coronary artery bypass graft surgery

Ahmadreza Jodati 1, Seyed Mohammadbagher Pirouzpanah 2,3, Nazila Fathi Maroufi 4,5, Masoud Pezeshkian 1, Naser Safaie 1, Hossain Bijanpour 2, Amir Mahdi Khamaneh 6, Ali Mota 2, Mohammad Nouri 1,2 * ORCiD

1 Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Stem cell and Regenerative Medicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
3 Lvts u1148 Inserm Institut Galilee Universite Paris 13, Paris, France
4 Department of Biochemistry & Clinical Laboratory, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
6 Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Introduction: microRNAs (miRNAs) are highly conserved, noncoding RNA molecules that regulate gene expression on the post-transcriptional level. Some evidence indicates that microRNAs dysfunction plays a crucial role in human disease development. The role of microRNAs in cardiac growth, hypertrophy, heart failure, cardiovascular complications in diabetes and many other hearth conditions are demonstrated. In this study we aimed to evaluate the expression of six microRNAs (mir-100, mir-126, mir-127, mir-133a, mir-133b and mir-145) that have been shown to overexpress in aortic and carotid plaques.

Methods: Thirty Coronary Artery Disease patients who underwent elective coronary artery bypass graft surgery were enrolled in the study. The expression patterns of six miRNAs (mir-100, mir-126, mir-127, mir-133a, mir-133b, and mir-145) were examined in 30 patients of whom we obtained aorta and saphenous vein samples.

Results: In three miRNAs, mir-100, mir-127 and mir-133b, we did not obtain expression data from real-time experiments. We found that the expression level of mir-126, mir-133a and mir145 were lower in aorta in comparison with saphenous vein. Mir-126 was highly expressed in saphenous vein samples (13.8±1.1) when compared with aorta samples (20.2±1.1), although mir133a was highly expressed in saphenous vein samples (16.1±0.5) when compared with the aorta (17.9±1.5). Expression of mir-145 saphenous vein samples was also dramatically higher than aorta (7.2±0.5 versus 10.8±0.6) that was statistically significant (P<0.05).

Conclusion: Understanding the role of miRNAs in cardiovascular physiology and diseases might suggest miRNA- based therapeutic methods in the management of coronary artery disease.

Please cite this article as: Jodati A, Pirouzpanah SM, Fathi Maroufi N, Pezeshkian M, Safaie N, Bijanpour H, Khamane M, Mota A, Nouri M. Different expression of Micro RNA-126, 133a and 145 in aorta and saphenous vein samples of patients undergoing coronary artery bypass graft surgery. J Cardiovasc Thorac Res 2019;11(1):43-47. doi: 10.15171/jcvtr.2019.07.
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