Logo-jcvtr
Submitted: 21 Mar 2022
Revision: 18 Jul 2022
Accepted: 18 Aug 2022
ePublished: 06 Sep 2022
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

J Cardiovasc Thorac Res. 2022;14(3): 172-179.
doi: 10.34172/jcvtr.2022.31
PMID: 36398045
PMCID: PMC9617054
Scopus ID: 85140378929
  Abstract View: 533
  PDF Download: 298
  Full Text View: 34

Original Article

Combination of polyglycerol sebacate coated with collagen for vascular engineering

Fateme Nazary Abrbekoh 1 ORCID logo, Nasrin Valizadeh 2, Ayla Hassani 3, Hakime Ghale 4, Soltan Ali Mahboob 1, Reza Rahbarghazi 5,6* ORCID logo, Ali Baradar Khoshfetrat 3, Mahdi Madipour 7

1 Department of Biochemistry, Higher Education Institute of Rab-Rashid, Tabriz, Iran
2 Department of Chemistry, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran
3 Chemical Engineering Faculty, Sahand University of Technology, Tabriz, Iran
4 Department of Polymer Science and Engineering, University of Bonab, Bonab, Iran
5 Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6 Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
7 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Corresponding Author: Reza Rahbarghazi, Email: , Email: Rezarahbardvm@gmail.com

Abstract

Introduction: Here, we monitored the cytocompatibility of scaffolds consisting of poly (glycerol sebacate) (PGS) coated with collagen (Col) for endothelial cell activity after 72 hours.

Methods: Human endothelial cells were allocated into Control, PGS, and PGS+Col groups. Scaffolds were characterized using FTIR and HNMR spectroscopy. Contact angel analysis and SEM were used to study wettability, surface morphology, and cell attachment. Cell survival was assessed using LDH leakage assay. Levels of Tie-1, Tie-2, VE-Cadherin, and VEGFR-2 were measured using western blotting and real-time PCR.

Results: FTIR and HNMR analyses revealed the proper blending in PGS+Col group. SEM imaging exhibited a flat surface in the PGS group while thin Col fibers were detected in PGS+Col surface. The addition of Col to the PGS reduced the contract angle values from 97.3˚ to 81.1˚. Compared to PGS substrate alone, in PGS+Col group, cells appropriately attached to the surface. PGS and PGS+Col did not alter the leakage of LDH to the supernatant compared to control cells, showing the cytocopatiblity of PGS-based scaffolds. SOD and NO levels were increased significantly in PGS (p<0.05) and PGS+Col groups (p<0.001), respectively. We found that PGS+Col decreased Tie-1 content in endothelial cells whereas protein levels of Tie-2 and VE-Cadherin and expression of VEGFR-2 remained unchanged compared to PGS and control groups.

Conclusion: Simultaneous application of Col and PGS can stimulate normal endothleial cell morphology without the alteration of tyrosine kinases receptors and cadherin.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 534

Your browser does not support the canvas element.


PDF Download: 298

Your browser does not support the canvas element.


Full Text View: 34

Your browser does not support the canvas element.