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Submitted: 28 Jun 2023
Accepted: 10 Feb 2024
ePublished: 13 Mar 2024
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J Cardiovasc Thorac Res. 2024;16(1): 28-37.
doi: 10.34172/jcvtr.32899
PMID: 38584661
PMCID: PMC10997974
  Abstract View: 247
  PDF Download: 322

Original Article

Acellular carotid scaffold and evaluation the biological and biomechanical properties for tissue engineering

Farina Rashidi 1* ORCID logo, Mehdi Mohammadzadeh 1, Arash Abdolmaleki 2 ORCID logo, Asadollah Asadi 3, Mehrdad Sheikhlou 4

1 Department of Biology, Faculty of Science, University of Urmia, Urmia, Iran
2 Department of Biophysics, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran
3 Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
4 Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran
*Corresponding Author: Farina Rashidi, Email: farinarashidi96@gmail.com

Abstract

Introduction: The issues associated with the limitation of appropriate autologous vessels for vascular reconstruction via bypass surgery highlight the need for new alternative strategies based on tissue engineering. The present study aimed to prepare decellularized scaffolds from ovine carotid using chemical decellularization method.

Methods: Ovine carotid were decellularized with Triton X-100 and tri-n-butyl phosphate (TnBP) at 37 °C. Histological analysis, biochemical tests, biomechanical assay and biocompatibility assay were used to investigate the efficacy of decellularization.

Results: Decellularization method could successfully decellularize ovine carotid without leaving any cell remnants. Scaffolds showed minimal destruction of the three-dimensional structure and extracellular matrix, as well as adequate mechanical resistance and biocompatibility for cell growth and proliferation.

Conclusion: Prepared acellular scaffold exhibited the necessary characteristics for clinical applications.

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