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Submitted: 01 Aug 2023
Revision: 10 Dec 2023
Accepted: 11 Dec 2023
ePublished: 30 Dec 2023
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J Cardiovasc Thorac Res. 2023;15(4): 244-249.
doi: 10.34172/jcvtr.2023.32926
PMID: 38357560
PMCID: PMC10862031
  Abstract View: 297
  PDF Download: 355

Original Article

Mechanical properties of the rabbit and human decellularized patches for well-tolerated/reinforced organ in cardiac tissue engineering

Maryam Mousavi Khatat 1 ORCID logo, Saeideh Same 2 ORCID logo, Keyvan Moharamzadeh 3, Jafar Soleimani Rad 1, Ahmad Mehdipour 1, Leila Roshangar 1,4* ORCID logo

1 Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
2 Research Center of Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
3 Hamdan Bin Mohammed College of Dental Medicine (HBMCDM), Mohammed Bin Rashid University of Medicine and Health Sciences (MBRU), Dubai, United Arab Emirates
4 Department of Anatomy and Histology, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Leila Roshangar, Email: lroshangar@yahoo.com

Abstract

Introduction: Natural decellularized patches have been developed as the therapeutic platform for the treatment of different diseases, especially cardiovascular disorders. Decellularized scaffolds (as both cell-seeded and cell-free patches) are broadly studied in heart tissue redevelopment in vivo and in vitro. The designed regenerative bio-scaffold must have desirable physicochemical properties including mechanical stiffness for load-bearing, and appropriate anatomical characteristics to mimic the native biological environment properly and facilitate tissue reconstruction. In this context, the current study was designed to investigate rabbit decellularized derma’s similarity with human decellularized skin in terms of mechanical properties for cardiac tissue engineering application.

Methods: Fifty two rabbit dermal specimens were provided and divided into two groups: the experimental (decellularized) group and the control (group). Similarly, twelve human skin specimens were divided into the experimental (decellularized) and control groups. Initially, the effect of decellularization on the mechanical performance of scaffolds was analyzed. Then, the mechanical strength of decellularized rabbit skin was compared to decellularized human derma by measuring the stress strain and Young’s modulus of the samples.

Results: The results showed that rabbit decellularized skin has a similar elastic range to human decellularized skin, despite being more elastic (P>0.05). In addition, after decellularization, both rabbit and human skin showed a non-significant decrease in elasticity (P>0.05). It is worth noting that the elasticity reduction in rabbit samples after skin decellularization was lower than in human samples.

Conclusion: According to the results of this study and the similarities of rabbit decellularized derm to human skin and its advantages over it, along with the biological complexity of native cardiac ECM, this scaffold can be used as an alternative matrix for tissue-engineered cardiac patches.

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