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Submitted: 10 Feb 2025
Revision: 09 Aug 2025
Accepted: 11 Sep 2025
ePublished: 17 Dec 2025
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J Cardiovasc Thorac Res. 2025;17(4): 274-282.
doi: 10.34172/jcvtr.025.33496
  Abstract View: 55
  PDF Download: 71

Original Article

Biochemical assessment of selenium’s cardiovascular protective effects in a lipopolysaccharide-induced damage in rats: Focus on oxidative stress markers and IL-6

Farimah Beheshti 1,2,3 ORCID logo, Mohammad Mahdi Sotoudeh 2,4, Mostafa Mansouri 4,5, Mohammad Mobin Mirimoghaddam 4, Yousef Baghcheghi 6* ORCID logo, Mahmoud Hosseini 4,7* ORCID logo

1 Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
2 Applied Biomedical Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Physiology, School of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4 Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
5 Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
6 Bio Environmental Health Hazards Research Center, Jiroft University of Medical Sciences, Jiroft, Iran
7 Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
*Corresponding Authors: Yousef Baghcheghi, Email: Baghcheghi@gmail.com; Mahmoud Hosseini, Email: hosseinim@mums.ac.ir

Abstract

Introduction: One of the main causes of illness and death in communities is cardiovascular disease (CVD). Inflammation and oxidative stress are key components in the pathophysiology of CVD. It has been demonstrated that selenium lowers inflammation and oxidative stress. The purpose of this study is to do biochemical assessment of selenium’s cardiovascular protective effects in a lipopolysaccharide (LPS)-Induced damage in rats.

Methods: LPS+Selenium (100 µg/kg), LPS (1 mg/kg), LPS+Selenium (200 µg/kg), and Vehicle (instead of both selenium and LPS) were given to the four groups of rats. The rats were sacrificed after 14 days, and the serum, heart, and aorta were examined for the presence of malondialdehyde (MDA), thiol, catalase (CAT), and superoxide dismutase (SOD). Interleukin 6 (IL-6) was also assessed in the tissues of the heart and aorta as an indicator of inflammation.

Results: LPS administration raised aortic and cardiac IL-6 levels (P<0.001). In the heart, aorta, and serum, it also raised MDA (P<0.001) and lowered thiol (P<0.001), CAT (P<0.01-P<0.001), and SOD (P<0.001). On the other hand, selenium therapy markedly raised thiol, CAT, and SOD levels (P<0.01-P<0.001) and lowered MDA levels (P<0.05-P<0.001). Furthermore, following selenium delivery, a decrease in the inflammatory marker IL-6 was noted (P<0.01-P<0.001).

Conclusion: This study showed that selenium protected the heart, aorta, and serum from oxidative stress brought on by LPS. Additionally, it reduced aortic and cardiac inflammation. These results imply that selenium’s anti-inflammatory and antioxidant properties may help prevent or lower the morbidity and mortality of CVD.


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